| Author(s) | El Fatih M. Mahdi, Hamad S. Al-Saad and Sakina M.A.I. Elshibili* |
| Affiliation | Plant Tissue Culture Laboratory, Department of Agriculture and Water Research, Ministry of Municipal Affairs and Agriculture P.O. Box 1967, Doha, Qatar |
| Title | In vitro Tuberization of Potato Cultivars as Influenced by Photoperiod, Exogenous Sucrose and Cytokinin Concentrations |
| Source | Journal of King Saud University. Agricultural Sciences. Volume 17, No 1. (2005/1425) |
| Abstract | The effect of cytokinin, sucrose and photoperiod on in vitro microtuber formation and growth of two potato cultivars. were evaluated. Uninodal stem segment explants were cultured on agar solidified Murashige and Skoog medium and tested at four BAP concentrations, four sucrose levels and four incubation photoperiods. Sucrose at 9% resulted in appreciably higher number of microtuber with heavier weights in Obelix cv., whereas, in Desiree both measurements increased progressively with the concentration. BAP induced early initiation of microtuber in Obelix cv. The best BAP concentration stimulates tuber formation and growth was 1mg/l. Nevertheless, both cultivars responded similarly in all other concentrations. With regard to the effect of photoperiodic treatments, large variations in tuberization were detected among photoperiods and genotype ranging from 0 to 70%. Significant differences in tuber number, size and weight were obtained at 14 LD +7 SD for Obelix cv. In Desiree on the other hand, dark treatment had a negative effect. |
