| Author(s) | Abdulhadi A. Alofi and Ahmed R. AlHimaidi* |
| Affiliation | King Saud University ,College of Science,Zoology Department. B.O. Box 2455, Riyadh 11451, Saudi Arabia |
| Title | In vitro Maturation(IVM) and Fertilization (IVF) of Sheep Ova Cultured in Two Different Media |
| Source | Journal of King Saud University. Agricultural Sciences. Volume 16, No 1. (2004/1424) |
| Abstract | The aim of this study is to produce an embryos from a sheep ova collected from ovaries at the slaughterhouse using two different culture media. About 305 ovaries were collected from 153 ewes at the slaughterhouse of Riyadh City. More than 1000 ova obtained from them, they were classified according to their morphological appearance into four groups. Only the ova with intact granulosa cells or filled cytoplasm (570 ova) were IVM in TCM-199 supplemented with FCS 10%, PMSG, LH and estrogen hormone After 29 hr., they were IVF with fresh diluted semen collected from a matured ram, in TALP medium. Only 57 (9.47%) of fertilized ova were cultured into two different culture media (TCM-199 from Sigma chemical company, and simple modified culture medium MCM prepared at our lab). In the TCM-199, 22 ova (81.48%) cleaved into a two cell stage, 18 of them (66,66%) became as a four-cell stage embryo, 13 of them (48.14%) developed to an eight-cell stage, only 6 embryos (22.22%) became as a sixteen cell stage, and only 4 of them reached the 32-cell stage and more ( morula stage). In the MCM, from the 27 fertilized ova 17 (62.96%) were cleaved to a two cell stage, 11 (40.47%) of them became as 4-cell stage embryo, only 7 (25.92%) cleaved to 8-cell stage, 4(14.81%) of them developed to 16- cell stage, and only 2 (7,4%) reached the 32-cell and more (the morula stage) . In conclusion, this study indicated that the complex culture medium is better twice as the simple culture medium, and the development of sheep embryo did require some vitamin, and amino acids for their early development in vitro. Keywords: In vitro maturation (IVM), fertilization (IVF) and culture (IVC), sheep embryo , modified culture medium (MCM), and TCM-199. |
